installation

  • Installation of vtamR package
  • Installation of third party programs
  • TaxAsssign data base

tutorial-vtamr-pipeline

  • How to construct a full metabarcoding pipeline
  • Supplementary functions
  • I/O files and data frames
  • Glossary
  • Trouble shooting

from-fastq-to-df

Quality filtering, merging, trimming demultiplexing, dereplication according to different fastq content.

  • One sample per fastq - no tag - no primer
  • One sample per fastq - primer - no tag
  • Several samples per fastq - tags - primers

make-mock-composition-file

How to pick the expected sequences in mock samples from your own data (in case you do not know them in advance).